Journal: Scientific Reports

Article Title: Membrane estrogen receptor α signaling modulates the sensitivity to estradiol treatment in a dose- and tissue- dependent manner

doi: 10.1038/s41598-023-36146-9

Figure Lengend Snippet: Loss of mERα signaling causes a right shift of E2 treatment responses. Tissue responses to different doses of E2 treatment in uterus ( A ), thymus ( B ), gonadal fat ( C ), tibia trabecular bone ( D ), and tibia cortical bone ( E ). X-axis represents the logarithmic mean value of serum E2 concentrations at termination (pg/mL). Y-axis represents the % response to E2 treatment calculated from % E2 response in WT mice in the high-dose experiment, which was set to 100%. All other % E2 responses were related to the % E2 response in WT mice from the high-dose experiment. The E2 response curve is visualized by using a sigmoidal, four parameter logistic (4PL) curve fitting model (GraphPad). Analyses of differences in E2 responses between WT and C451A were done by two-way ANOVA. All individual values are presented with mean (horizontal line) and SEM (vertical lines). ** p < 0.01, *** p < 0.001, conc.; concentration, BV/TV; bone volume per tissue volume, Ct. Th.; cortical thickness.

Article Snippet: The E2 response curve is visualized by using a sigmoidal, four parameter logistic (4PL) curve fitting model (GraphPad).

Techniques: Concentration Assay